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I had my genome sequenced!

Sandy Braille said:
Keep in mind that sequencing using both Single Nucleotide Polymoprhisms and Short Tandem Repeats isn't as accurate as you'd think. Whole Genome Sequencing is in a completely other echelon than STR analysis, as using relativism with short polymorphisms definitely has its limitations.



https://www.cstl.nist.gov/strbase/pub_pres/FSMP_STRs_vs_SNPs.pdf


Still cool though Siromar!!
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Sandy sandy sandy......

Siromar had his/her genome sequence... Meaning every gene was determined which allele they carry...

What you are talking about and the article you mentioned is talking about is the difference in SNPs and STRs in genetic testing when trying to match the DNA of two individuals, like when doing paternity tests, or when comparing DNA samples from a crime science and a suspect in the crime!!! In each case actual genes aren't sequenced, where Siromar had all his/her genes sequenced!!!



Uhg..... Students!
 
moevillini said:
beats having sex, eh?
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I'm trying to wrap my head around what's upsetting you. The best explanation I can think of is that you misinterpreted a fraction of sentence that I wrote as some kind of anti-sex sentiment. Am I close?

If that is the case, you just misinterpreted me. In fact, I'm willing to bet that I have less problem with sex than yourself or anyone you know. I'm THAT confident.
 
Zulu said:
Sandy sandy sandy......

Siromar had his/her genome sequence... Meaning every gene was determined which allele they carry...

What you are talking about and the article you mentioned is talking about is the difference in SNPs and STRs in genetic testing when trying to match the DNA of two individuals, like when doing paternity tests, or when comparing DNA samples from a crime science and a suspect in the crime!!! In each case actual genes aren't sequenced, where Siromar had all his/her genes sequenced!!!



Uhg..... Students!
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A biff on the article selection, perhaps. Forgive me, master.

Also: his genome was sequenced? I thought it was simply genotyped...


At its most basic level the human genetic sequence is a three-billion letter string of A's, T's, G's and C's (the code of DNA). Most of that sequence is the same in everyone. But there are an estimated 10 million places in the genome where a single letter of the sequence sometimes differs from one person to the next.

Those one-letter spelling variations are known as SNPs, or single nucleotide polymorphisms, and they form the basis for the genotyping used by 23andMe. A person who has a "C" at a particular spot, for example, might be more sensitive to bitter tastes than a person with a "G" in that spot.

In all, 23andMe looks at 1,000,000 SNPs scattered across the 23 pairs of chromosomes that constitute the human genetic sequence. We also look at a few thousand places on the mitochondrial DNA, an odd loop of genetic material outside the nucleus that is involved in producing energy for the cell.

The genotyping technology we've chosen is referred to as a BeadChip and although the process relies heavily on massive computer power, the chip itself is not a microprocessor but a miniaturized genetics lab. The BeadChip is a small glass slide with millions of tiny beads on its surface. Attached to each bead are probes - bits of DNA complementary to sites in your genome where SNPs of interest are located. Your DNA will stick to the probe that matches whichever SNP you happen to have.

In the genotyping process, a person's DNA is chopped up into pieces and washed over the chip, where each fragment sticks to any probes that are complementary to it. Then fragments of DNA that have been specially tagged are introduced to the chip in such a way that they stick to any probes that are themselves paired perfectly with sample DNA. At that point, the tagged DNA fragments are triggered to glow indicating which version of each SNP is present in the sample. It's a scientifically complex process, but a simple and reliable method in practice! The technology that we use, the Illumina OmniExpress Plus, analyzes approximately 1,000,000 SNPs that cover the entire genome. Although this is still only a fraction of the more than 10 million SNPs that are estimated to be in the human genome, these 1,000,000 are specially selected because they provide a lot of information about other nearby SNPs. This maximizes the information we can get from every SNP we analyze, while keeping the cost low.


In addition, we have hand-picked more than 30,000 additional SNPs of particular interest from the scientific literature and added them to the chip. As a result, we can provide you with unique, genetic information available through no other service. If you would like to view more details on Illumina's genotyping platform, please visit the Illumina website.
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And, most importantly,

So why we don't just sequence everyone's entire genome, and find every single genetic variant they possess? Unfortunately, sequencing technology has not yet progressed to the point where it is feasible to sequence an entire person’s genome quickly and cheaply. It took the Human Genome Project over 10 years' work by multiple labs to sequence the whole genomes of just a few individuals.

For those with specific needs (undiagnosed medical issues, for example, or a burning curiosity and a generous budget), sequencing will become a more and more attractive option. For now, though, genotyping technologies such as those used by 23andMe provide an efficient and cost-effective way of obtaining more than enough genetic information for scientists—and you—to study.
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Sandy Braille said:
A biff on the article selection, perhaps. Forgive me, master.

Also: his genome was sequenced? I thought it was simply genotyped...




And, most importantly,
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I understood that the whole genome wasn't being sequenced... I assumed they just sequenced all known genes in your genome.... When I looked on the website I didn't find the exact info... But thanks for finding it for me!! I'll be sure to say genotyping instead of genome sequencing next time!!

Anyways I'm looking forward to having this done...

I wonder if you can request certain genes to be genotyped..

I would like to see what my VMAT2(God Gene)looks like..

And my CCR5 gene... To see if I'm immune to HIV!!
 
Zulu said:
I doubt they give this information... Mainly Cuz it's junk DNA, but it would be awesome to know!!
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It is junk DNA but the reason we age is our telomeres are shrinking. If our telomeres never shrink we would never die from getting old. It would be the first thing I want to know about my DNA.
 
The Black Swordsman said:
It is junk DNA but the reason we age is our telomeres are shrinking. If our telomeres never shrink we would never die from getting old. It would be the first thing I want to know about my DNA.
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That's not entirely true. Telemere shortening does eventually cause the DNA to be exposed and breakdown. But death is very complex and multi-faceted. Even if you eliminate all the usual suspects like telomeres, oxidation, and metabolic stress, you'd still die thru other mechanisms. It's been a couple of years since I've seriously read on the subject, but I remember there was 7 major mechanisms that result in the eventual death of the organism.

Either way, this was just an exome sequence. Telomeres weren't touched.
 
The Black Swordsman said:
It is junk DNA but the reason we age is our telomeres are shrinking. If our telomeres never shrink we would never die from getting old. It would be the first thing I want to know about my DNA.
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I sure hope you posted this for those who didn't know what telomeres are and not that I didn't??


SiroMar said:
That's not entirely true. Telemere shortening does eventually cause the DNA to be exposed and breakdown. But death is very complex and multi-faceted. Even if you eliminate all the usual suspects like telomeres, oxidation, and metabolic stress, you'd still die thru other mechanisms. It's been a couple of years since I've seriously read on the subject, but I remember there was 7 major mechanisms that result in the eventual death of the organism.

Either way, this was just an exome sequence. Telomeres weren't touched.
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I do think if you could extend telomeres in your constant dividing cells that you could put off the grave a few extra years, but I think no matter what degradation of non dividing cells will ultimately bring on death!!

Read this article about a supplement to extend telomeres!!

https://www.scientificamerican.com/article.cfm?id=anti-aging-pill-targets-telomeres
 
Zulu said:
I sure hope you posted this for those who didn't know what telomeres are and not that I didn't??




I do think if you could extend telomeres in your constant dividing cells that you could put off the grave a few extra years, but I think no matter what degradation of non dividing cells will ultimately bring on death!!

Read this article about a supplement to extend telomeres!!

https://www.scientificamerican.com/article.cfm?id=anti-aging-pill-targets-telomeres
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I think it was directed towards you. The Black Swordsman is in premed, so obviously his opinion trumps your shovel ware degrees and Dixie expertise.
 
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